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Antimicrobial peptides of entomopathogen-challanged superworm Zophobas morio Col.: Tenebrionidae

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عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran,
چکیده
Insect immunity relies on cellular and humeral innate mechanisms. An important role in humeral immune response against pathogens is played through phenoloxidase activity as well as expression of antimicrobial peptides or proteins (AMPs). Due to the promising activity of insect AMPs, attempts are being made to use these peptides as antimicrobial agents. AMPs production as a part of Z. morio immune responses have not been examined so far. Our study shed light on antimicrobial peptides of Z. morio larvae challenged with Beauveria bassiana and Bacillus thuringiensis, as the most important entomopathogens. The Z. morio larvae have been treated by B. bassiana and B. thuringiensis suspensions. The haemolymph of larvae was collected 6, 12, 24, 48 and 72 hours post-injection. The larval haemolymph has been extracted and the protein profile was identified following SDS-PAGE and NATIVE-PAGE analyses. As a result, a distinct band corresponding to insect AMP was detected in the haemolymph of immune-challenged Z. morio larvae with both bacterial and fungal pathogens. Following the extraction of detected peptides from NATIVE-PAGE, the inhibitory zone assay was carried out. The antimicrobial activity of extracted AMP was assessed in vitro against B. thuringinesis and B. bassiana as insect pathogens, and Fusarium graminearum as phytopathogen. Based on our findings, the extracted peptides had a great antibacterial activity against the tested pathogens. In summary, it can be concluded that Z. moroi larvae immune system respond to insect bacterial and fungal infections, therefore superworms can be used as an alternative model to study insect antimicrobial peptides.

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