Investigation on resistance to abamectin in Iranian populations of western flower thrips, Frankliniella occidentalis (Thys.: Thripidae)
پذیرفته شده برای پوستر
عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران
نویسندگان
1Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran, zgholami
2Department of Pesticide Research, Iranian Institute of Plant Protection, AREEO, Tehran, Iran,
چکیده
In current study resistance to abamectin was detected and confirmed in greenhouse populations of western flower thrips, Frankliniella occidentalis from eight areas of Iran including Tehran, Markazi, Alborz, Qazvin, Isfahan, Yazd M, Yazd B and Kerman. As a first step to understand the level of resistance, LC50 value of abamectin on 2nd instar larvae was estimated by green bean pod sections-dipping method. At least five concentrations and three replicates were used per concentration. Mortality was recorded 48 h after treatment. Results of dose-mortality bioassays revealed resistance ratios ranging from 13.19 to 59.57-fold for abamectin. Elevated LC50 value was obtained from Alborz strain with the highest resistance ratio when compared to the susceptible population of Isfahan. Synergism experiments with piperonylbutoxide (PBO) (P450 inhibitor) and S,S,S-tributyl-phosphorotrithioate (DEF) (esterase inhibitor) in a concentration of 1000 mg/L performed to demonstrate the role of metabolic resistance. Based on the results, PBO reduced the resistance ratios for seven populations although there was a higher reduction in resistance of Alborz population exposed to TPP (4.88 fold). These results suggest the role of the detoxifying enzyme, multifunction oxidases (MFO), might be involved as a resistance mechanism. We suggest that abamectin should be used with cautiousness in the management program of western flower thrips.
کلیدواژه ها