Integrative taxonomy of some West Palearctic Trichogramma species

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عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران

¹INRA, Institut Sophia Agrobiotech, Sophia Antipolis, France,

²INRA, Centre de Biologie pour la Gestion des Populations, Montpellier, France,

Trichogramma are minute chalcid wasps that are, altogether, egg parasitoids of various insect orders (mainly Lepidoptera). The genus is extremely diverse with more than 200 described species worldwide and 40 in Europe. This diversity, their small size as well as the possible presence of species complexes make difficult their identification, in particular by non-specialists. This situation is detrimental not only for basic research but also (and maybe more importantly) for the use of some Trichogramma strains as biological control agents. Using (i) intensive DNA Barcoding using part of the mitochondrial marker Cytochrome Oxidase I (COI), (ii) multi-locus phylogeny including COI but also other mitochondrial (CytB) and nuclear markers (Wingless, EF1alpha, RpL27a et RpS4), (iii) crossing experiments as well as (iv) additional sources of information (e.g. previous identifications based on morphological characters or GENBANK accessions), we investigated the diversity of Trichogramma strains collected by ourselves or provided by various international colleagues in the Western Palaearctic. Taken as a whole, we hence identified about 20 Molecular Operational Taxonomic Units (MOTUs), most of them being cross-validated by other sources of information. Among this diversity, some MOTUs were nevertheless not associated with a species’ name and required further investigations. Moreover, two species complexes were observed, the first one encompassing T. cacoeciae and T. embryophagum as well as putative new species and the second one encompassing some strains previously identified as T. brassicae and T. euproctidis. Based on this first dataset, our perspective is to widen our collaborative network not only to have access to new strains/information but also to unite disseminated specialists of this genus. This objective is also supported by the creation and certification (ISO9001:2015) of a Biological Resource Center in France devoted to Egg Parasitoids (BRC EP-Coll – https://www6.inra.fr/crb-eggparasitoids-coll/Presentation).

Keywords: Biodiversity, Biological control, Integrative Taxonomy, Species delineation, Trichogramma.

Biodiversity؛ biological control؛ Integrative Taxonomy؛ Species delineation؛ Trichogramma