Nanoemulsions of eucalyptus essential oil against Tribolium castaneum
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عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Department of Plant Protection, Faculty of Agriculture, Faculty of Agricultural Sciences and Natural Resources, University of Khuzestan, Mollasani, Ahvaz, Iran,
چکیده
The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is one of the most important pests of grain storage products. Chemical control using synthetic insecticides and fumigants is the primary method for managing the pest which has resulted in serious adverse effects on mammals, other non-target organisms and environment. Efforts have been focused on botanical products essential oils (EOs) as possible alternative to conventional insecticides. Some limitation including low physical stability, poor water solubility, and quick degradation in the environment have made EOs application impractical. Nanotechnology method including nanoformulations is a good strategy to overcome the mentioned limitations. In this study, contact toxicity of Eucalyptus globulus Labill essential oil compared with its nanoemulsion was investigated on T. castaneum adult. In preparation of nanoemulsion, Span® 80 and gum arabic were used via ultrasonic emulsification method. Results indicated that highest toxicity (LC50 =6.82 ppm) was related to pure essential oil after 24 h and LC50 of two nanoemultions had no significant difference. After a 48 h exposure period, the LC50 values of Span® 80, gum arabic nanoemulsion and pure EO were 15.05, 5.55 and 5.02 ppm, respectively. Also, after 72 h exposure period, the Span® 80 nanoemulsion had the highest value (4.96 ppm), whereas the pure EO and gum arabic nanoemulsion values were 6.17 and 11.04 ppm, respectively. Overall, our findings indicated that Span® 80 nanoemulsion improve the stability of E. globulus essential oil increasing its efficacy against T. castaneum and this could be considered in the integrated pest management program.
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