Effect of citrus leaf-miner, Phyllocnistis citrella Stainton (Lep.: Gracillaridea) on chlorophyll and carotenoids of different species of citrus
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عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
Determining biochemical traits of different cultivars can help to identify effective mechanisms for resistance of plants to pests. The citrus leaf miner, Phyllocnistis citrella Stainton, is one of the most common citrus pests in nurseries and young orchards, causing heavy damage every year. In the current study, four citrus species including Orange (Citrus sinensis L.), Key lime (C. aurantifolia L.), Sweet lemon (C. limetta L.) and Sour orange (C. aurantium L.) located in the citrus garden of Department of Plant Protection, Shahid Chamran University of Ahvaz, were selected for the experiments. To evaluate the impact of citrus leaf-miner damage on the pigment content of citrus leaves, sampling of damaged and healthy leaves (as control) was carried out randomly. The leaves were placed into a plastic bag and transferred to the lab. First, leaves were washed with distilled water and air-dried. Then, spectral absorption, UV-Vis Spectrophotometer was used to evaluate the amount of chlorophyll a, b, total and carotenoids. Statistical analysis was done in a completely randomized design with three replications and mean comparison was performed with Tukey's test by SPSS 16.0. The results showed that there was a significant difference between control and damaged levels for all measured parameters. The levels of chlorophyll a, b, total and carotenoids in damaged leaves of all species significantly decreased compared to those in healthy leaves. The highest mean of chlorophyll a was in healthy Key lime (0.58 ± 0.04 mg / g) and healthy orange (0.53 ± 0.02 mg / g) and the lowest was observed in infested Sweet lemon (0.22 ± 0.02 mg / g). Comparison of mean chlorophyll b showed that the highest mean was recorded in healthy oranges (0.18 ± 0.02 mg / g) followed by healthy Key lime (0.15 ± 0.01 mg / g), which were statistically significant, while the lowest amount of chlorophyll b was in infested orange (0.05 ± 0.01 mg / g). The highest and lowest chlorophyll total content were for healthy leaves of Key lime (0.73 ± 0.06 mg / g) and infested Sweet lemon leaves (0.29 ± 0.02 mg / g), respectively. Also, the carotenoid content in healthy leaves of Key lime was the highest amount (0.30 ± 0.01 mg / g) and the lowest carotenoid was in infested leaves of Sour orange (0.10 ± 0.00 mg / g). It could be concluded that in the damaged plants the content of chlorophyll a, b, total and carotenoid were significantly reduced in compared with the their amount in control plants.
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