Insecticide resistance of Anopheles stephensi Liston (Diptera: Culicidae) to conventional pesticides in a malaria endemic area of southern Iran
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عنوان دوره: سومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Madineh Abbasi1 ، Ahmad Ali Hanafi-Bojd1 ، Moammad Reza Yaghoobi-Ershadi2 ، Hassan Vatandoost3 ، Mohammad Ali Oshaghi1 ، Teimour Hazratian4 ، Mohammad Mehdi Sedaghat1 ، Sajjad Fekri5 ، Reza Safari6 ، Abdol Rasoul Mojahedi6 ، Yousef Salari Salari6
1Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran,
2Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, Department of Environmental Chemical Pollutants and Pesticides
4Department of Parasitology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5Department of Diseases Control Hormozgan University of Medical Sciences, Bandar Abbas, Iran,
6Department of Diseases Control, Hormozgan University of Medical Sciences, Bandar Abbas, Iran,
چکیده
The aim of this study was to evaluate the sensitivity of Anopheles stephensi (the main malaria vector) to common chemical pesticides in plane areas of Bandar Abbas County, Southern Iran. Larvae of An. stephensi were collected from different larval habitats in the urban area of Bandar Abbas city and Hormoodar village. According to World Health Organization guideline, diagnostic dose of insecticides was used against female adult and larvae of An. stephensi mosquitoes. The used insecticides were: Bendiocarb 0.1% (Batch No.159, Expiry date: March 2019), Permethrin 0.75% (Batch No. 428, Expiry date: August 2018), Lambda-cyhalothrin 0.05% (Batch No. 262, Expiry date: July 2018) and Deltamethrin 0.05% (Batch No. 527, Expiry date: August 2018). The control test mosquitoes were exposed to papers impregnated only with the appropriate carrier oil for Pyrethroids (Batch No. 262, Expiry date: August 2018) and carbamates (Batch No. 181, Expiry date: July 2020); that was without insecticide. Tests were performed by the F1 progeny of wild-caught adult females An. stephensi aged 3–5 days that were sugar fed. Two larvicides, i.e. Temephos (0.25mg/l) and Chlorpyriphos (0.025mg/l) were used at the diagnostic dose provided by WHO. Collected larvae from breeding places were transferred to insectary of Bandar Abbas Research Station. After 24 hours recovery time, late 3rd or early 4th instars were used for the tests by WHO test procedure for mosquito larvae Tests were carried out in an insectary maintained at 27 ±2 °C temperature and 75±10% relative humidity. Results showed that both populations of An. stephensi were completely susceptible to temephos and tolerant to chlorpyriphos with Mortality Rate from 97 to 100 percent in the diagnostic dose recommended by WHO. This mosquito showed low resistance level to lambda-cyhalothrin and deltamethrin with Mortality Rate respectively 84.31±2.62 and 89.66 ±2.34, and tolerance to bendiocarb and permethrin with Mortality Rate respectively 91.3±2.15 and 92.16 ±1.93 in Hormoodar population. Widely use of pyrethroids in agriculture is certainly effective on increasing resistance to pyrethroids. The intersectional coordination and collaboration in health and agriculture seems to be necessary to reduce the mosquitos’ resistance.
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