Biochemical basis of DDT resistance in Anopheles stephensi (Diptera: Culicidae) in southeastern of Iran
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Mohammad Amin Gorouhi ، Mohammad Ali Oshaghi ، hassan vatandoost ، Ahmad Ali Enayati ، Abbas AghaeiAfshar ، Ahmad Ali Hanafi-Bojd ، fatemeh kamiabi ، yaser salim abadi ، Alireza Sanei-Dehkordi ، Azim Paksa
چکیده
Introduction: Anopheles stephensi (Diptera: culicidae) is a key urban malaria vector in the Indian subcontinent and Middle East including south and southeast of Iran. Wide distribution of DDT resistant in Anopheles stephensi populations engage us to find underlying resistant mechanism which can be helpful in resistant management programs.
Material and Methods: Mean activity of each enzymes including cytochrome P450s, alfa- esterase, beta_ esterase, glutathione S transferases (GSTs) were measured in selected population (resistant ratio=2.05 ) and the results were compared with those of the susceptible Beech strain using the World Health Organization approved biochemical assay for the adult mosquitoes.
Results: Enzymes activity significantly varied in the resistant strains of Chahbahar as compared with the susceptible strain. The cytochrome P450s enzyme ratios were 9.13 fold in the DDT resistance population compared with the susceptible strain. The enzyme ratios for alpha-esterase and beta-esterase were 3.95 and 2.40 respectively also it was 4.72 in case of GSTs enzyme compared with the susceptible strain.
Discussion: Elevation of enzymes especially cytochrome P450s play a crucial role in the development of DDT resistance in An. stephensi. Therefore, evaluation of synergists in order to deactivate the metabolic mechanisms in resistant management is highly suggested. It’s not recommended application of insecticides with the same mode of action.
Material and Methods: Mean activity of each enzymes including cytochrome P450s, alfa- esterase, beta_ esterase, glutathione S transferases (GSTs) were measured in selected population (resistant ratio=2.05 ) and the results were compared with those of the susceptible Beech strain using the World Health Organization approved biochemical assay for the adult mosquitoes.
Results: Enzymes activity significantly varied in the resistant strains of Chahbahar as compared with the susceptible strain. The cytochrome P450s enzyme ratios were 9.13 fold in the DDT resistance population compared with the susceptible strain. The enzyme ratios for alpha-esterase and beta-esterase were 3.95 and 2.40 respectively also it was 4.72 in case of GSTs enzyme compared with the susceptible strain.
Discussion: Elevation of enzymes especially cytochrome P450s play a crucial role in the development of DDT resistance in An. stephensi. Therefore, evaluation of synergists in order to deactivate the metabolic mechanisms in resistant management is highly suggested. It’s not recommended application of insecticides with the same mode of action.
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