Effect of phytoecdysteroid extract of Dianthus orientalis and Silene aucheriana on enzyme activities and energy reserves of Plutella xylostella (L).

عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران

Mina Kouhjani Gorji؛ Saeid Moharramipour

The Phytoecdysteroids are molting hormone analogues in insects, which act by ingestion and contact with body surface interfere in intermediary metabolites through disruption in development, reproduction and eventually reduction of insect pest population. The experiments were designed to study the effects of phytoecdysteroid extract of Dianthus orientalis and Silene aucheriana on Some energy reserves and biochemical metabolites in third-instar larvae of the diamondback moth (DBM) Plutella xylostella (L)., that fed for two days in controlled conditions (27 ± 1ºC, 65±5% RH and a photoperiod of 16:8 (L: D) h). At first, ecdysteroidal fraction of extract was separated and then, leaves of canola were exposed to constant concentrations of 2, 5, 10, 20 and 40%. The activity of Aspartate Amino transferase (AST), Alanine Amino transferase (ALT), Alkaline Phosphatase (ALP), α-amylase, total fat, soluble protein, triglyceride, and uric acid for treated larva were measured. After four days the rate of total fat and soluble protein were reduced, but uric acid was increased. Similarly, the activity of AST, ALP and α-amylase was reduced as concentration increased. Hence some part of growth disorder and reduced fecundity that were observed may be ascribed to the created metabolic disorders in the given insect. Our finding suggests that the phytoecdysteroid extracts can be efficiently used as a botanical insecticide.

Phytoecdysteroid؛ Dianthus orientalis؛ Silene aucheriana؛ Plutella xylostella؛ enzyme activities؛ Energy reserves