The effect of thermal stresses on the immune system of the Potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
The hemocytes of insects are one of important components of immune system of insects against various stresses such as pathogens attack, parasitoids, starvation period and temperature changes. Hemocytes characteristics recognition and frequency in cellular Immune studies will help us in order to better pest control. In this study hemocytes of fourth instar larvae of Phthorimaea operculella (Zeller) were identified after staining with Giemsa and by light microscopy at 40x magnification. 5 types of hemocytes in hemolymph of Potato tuber moth were identified that included the prohemocytes (PRs), plasmatocytes (PLs), granulocytes (GRs), oenocytoids (OEs) and spherulocytes (SPs). The effect of different thermal stresses also were investigated for 24 hours on cellular defense of fourth instar larvae of the Potato tuber moth. In addition Number of various hemocytes and total number of blood cells were investigated. The results showed that total hemocyte count (THC) and PLs of larvae in which were for 24 hours under temperature stress of 35 ° C, increased significantly compared to the control temperature (25±1 ° C). Also in the effect of chill stress (4 ° C) on THC, PLs and OEs showed a significant decrease compared to the control. The identification of hemocytes of P. operculella and the effect of temperature on its frequency has been conducted for the first time and could be used as a base for further investigation on the immunology studies of this insect.
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