Assessment of host-associated population variations of green oak leaf roller moth, Tortrix viridana (Lep.:Tortricidae) by using ISSR markers in the northern Zagros forests (Iran)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
The green oak leaf roller Tortrix viridana L. is one of the most important pests in oak forests including Quercus infectoria, Q.branti, and Q.libani in northern Zagros. The larvae by feeding of leafs and leaf buds causes the tree to have no leaves in the spring. This state has a profound effect on the health of the tree. In This study, we assessed genetic diversity of T.viridana on three considered host plants by use of six polymorphic Inter simple sequence repeat (ISSR) markers, which produced reproducible banding patterns. One hundred and seventy-one individuals were collected from 9 different regions including baneh, sarvabad, bashmagh, gamarelang, Dezli and seif in Kurdistan province and sardasht, mirabad and piranshahr from west Azarbayjan province. In each region from each host, about 9 individuals of T. viridana larvae and pupae were collected (in baneh only 9 sample from Q. branti and in sarvabad only 9 sample from Q. infectoria were collected, piranshahr, gamarelang and bashmagh included each three host tree and other region included Q. branti and Q. infectorica). The immature stages of the moths were reared to adults in the laboratory and then adults were shock-frozen and stored at 20°C until DNA extraction. DNA extracted by use ATMAB protocol following PCR amplifications were carried out. To analyze the PCR product, 5 µL of product was loaded in a 2% agarose gel. Presence or absence of bands was scored as a 1 or a 0, thus our data was consisted by a presence/absence (1/0) matrix of 665 multiple bands from 171 individuals. The GenAlEx 6.502 software was used to calculated genetic diversity, genetic identity, band frequency, genetic distance principal coordinate analysis PCoA and Analysis of molecular variance (AMOVA). Dendrogram was constructed using UPGMA, Dice similarity coefficient and Nei genetic distance by XLSTAT software. We observed 640 polymorphic loci across the 171 individuals sampled. Shannon index was 0.249. The observed genetic distance (Nei H) for pest collected from three host was 0.006 (Q. brantii -Q. infectoria), 0.015 (Q. brantii-Q. libani) and 0.013 (Q. brantii -Q. libani). Maximum amount of heterozygote was 0.163 for Q. libani. Clustering analysis revealed strong concordance between the pest and host plants. based on Nei’s genetic distance, dendrogram was formed with 10 group. Analysis of molecular variance demonstrated that 8% of the total variability (FST = 0.08, P < 0.001) was attributable to variation among 3 population were collected from same host.
کلیدواژه ها