Assessment of hemolytic activity of the body extracts Ectomyelois ceratoniae
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
Hemolysis is the premature destruction of erythrocytes. The hemolysis assay is used to determine the hemolytic effect of a test compound. Hemolytic activity should be investigated in the process of identifying antimicrobial peptides of insects and future therapeutic applications. In this study, for hemolytic activity of the body extracts (the extracts Ultrafiltration membrane of 10 kDa was passed) larvae Ectomyelois ceratoniae (Zeller), 5 ml of fresh human, sheep and hen blood was added to a heparinized tube. The hemolytic assay was then carried out according to the methods reported in previous studies. The absorbance at 567 nm was then measured. Triton X-100 (0.1%), which yields 100% hemolysis, was used as a positive control. The results were compared with the control. Human and sheep red blood cells showed good tolerance while exposed to the body extracts larvae E. ceratoniae. The body extracts had no significant hemolytic effect in the concentration range (0.5–2 mg/ml) against human and sheep blood, but had a little hemolytic effect against hen blood. The results showed that hemolysis activity varies according to blood type.
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