Genetic diversity of some Japanese races of silkworm Bombyx mori by ISSR (inter-simple sequence repeat) molecular marker
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
Identification of biodiversity in the silkworm is also important for designing better Breeding programs Genetic diversity of five Japanese races of silkworm Bombyx mori stored in Iran Silkworm germplasm were studied by molecular marker ISSR. Silkworm races reared in Iran Silkworm Research Center (Rasht, Iran) according standard methods. Six adults collected for DNA extraction in order to study genetic diversity. PCR products loaded on 1.5% agarose gel for 3 h in 80v and stained by ethidium bromide for visualizing by gel documentation. Number of monomorphic and polymorphic produced fragments, polymorphism percentage, polymorphic information content, marker index, resolving power, effective multiplex ratio were calculated. Cluster analysis of five population obtained by NTSYS 2.01 software. Results showed twelve used primers produced 156 scorable bands with fragment size between 250-3000 bp. Obtained dendrogram inserted studied populations in three various groups accordance with geographical origin. It seems that ISSR molecular marker could discriminated geographical races of Japanese lines.
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