Identification of first foraging age loci in Iranian honeybee (Apis mellifera meda)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Gholamhosein Tahmasbi؛ Siavash Afraz؛ Alireza Tarang؛ Seyyedeh Azadeh Javadi Taklimi؛ zahra Tahmasbi؛ Rahim Saighalani؛ payam potki
چکیده
Honeybee is a pollinator that has prominent role in agriculture products and nourishment for human. So, the most number of studies like genetic improvement were done on honeybee. Recent advances in knowledge of molecular genetics of bee emphasize the potential for using genetic markers to select favored traits. This research was done to determination of first foraging age (AFF) loci's in Iranian honeybee (Apis mellifera meda). In the first phase, two colonies with high production- long longevity and low production- short longevity were selected from 11th generation of “Iranian Honeybee Breeding Project. Considering that colonies with different genotypes have different foraging, Iranian honey bee colonies were compared in foraging behavior using two high and low honey production colonies and their F1 backcrosses colonies.
In the high production colony, foraging initiation had started sooner (age of 9-33 day).Also‚ the length of foraging initiation period in comparison with low production colony (age of 12-29 day) was longer. In the end of first and second phase, worker bees of high and low production colonies after numbering and determining of foraging initiation, were analyzed through AFLP method for appointment of loci polymorphism. The results of molecular analyzes in parent and backcrosses colonies were used for determining of foraging initiation loci in Iranian honeybee.
There is a correlation between foraging behavior, performance and appearance traits with honey production in honeybee colonies. Determination of honey production characteristics provide sometimes help beekeepers and researchers with identifying high-producing colonies effectively and over less time. In mapping of quantitative trait loci (QTL), the relationship between one or more markers and QTL is studied. Therefore, we constructed an amplified fragment length polymorphism (AFLP) genetic linkage map of honey bee using 46 progenies from a BC population of the crossing between a queen from high production colony and a male from low production colony, selected from eleventh generation of honeybee breeding program. Data were analyzed using the software Win QTL Cartographer and Map Manager Ver. 2.6.5. Total 113 polymorph bands formed by 30 MseI / EcoRI primary combinations were classified in 8 linkage groups, and the length of the whole map was 4436.5 centimorgans, and average distance between markers was 8 centimorgans. Interval mapping identified 2 QTLs controlling foraging behavior which were in linkage groups 1 and 3.
In the high production colony, foraging initiation had started sooner (age of 9-33 day).Also‚ the length of foraging initiation period in comparison with low production colony (age of 12-29 day) was longer. In the end of first and second phase, worker bees of high and low production colonies after numbering and determining of foraging initiation, were analyzed through AFLP method for appointment of loci polymorphism. The results of molecular analyzes in parent and backcrosses colonies were used for determining of foraging initiation loci in Iranian honeybee.
There is a correlation between foraging behavior, performance and appearance traits with honey production in honeybee colonies. Determination of honey production characteristics provide sometimes help beekeepers and researchers with identifying high-producing colonies effectively and over less time. In mapping of quantitative trait loci (QTL), the relationship between one or more markers and QTL is studied. Therefore, we constructed an amplified fragment length polymorphism (AFLP) genetic linkage map of honey bee using 46 progenies from a BC population of the crossing between a queen from high production colony and a male from low production colony, selected from eleventh generation of honeybee breeding program. Data were analyzed using the software Win QTL Cartographer and Map Manager Ver. 2.6.5. Total 113 polymorph bands formed by 30 MseI / EcoRI primary combinations were classified in 8 linkage groups, and the length of the whole map was 4436.5 centimorgans, and average distance between markers was 8 centimorgans. Interval mapping identified 2 QTLs controlling foraging behavior which were in linkage groups 1 and 3.
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