Influence of red kidney bean seed proteins on larval gut protein pattern of Colorado potato beetle, Leptinotarsa decemlineata (Say)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
In this study, the impact of ingested red kidney bean, Phaseolus vulgaris cv. Goli (L.), seed proteinaceous extract on the larval gut protein pattern of Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say) were investigated by two-dimensional electrophoresis (2-D electrophoresis) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the 2-D electrophoresis, proteins were separated in two dimensions based on isoelectric point (pI) and molecular weight. For this purpose, the gut samples of larvae reared on the bean seed extract treated leaves of Picasso potato cultivar and related control, in which leaves treated with distilled water, was used. After electrophoresis, the gels were silver stained. The experiment was repeated five times. According to the results, from a total of 107 protein spots in the control and treatment, seven spots in the control (6.54%) and 29 spots in the treatment (27.10%) were unique spots, and 71 spots (66.36%) were identical in two gels. Most of added spots in treatment were observed in the isoelectric points 5 and 6. Also, general influence of seed extract and potato cultivar on gut proteins was detected by SDS-PAGE. In this investigation, the larval gut extract reared on treated leaves of four cultivars of potato (Agria, Burren, Picasso and Marx) with red kidney bean seed extract and distilled water (as control) was used. SDS-PAGE was performed using 12% stacking and 4% resolving gels and the gels were transferred to coomassie brilliant blue R-250 stain solution. A number of protein bands were different between control and treatment, and also between the different cultivars clearly at bands with molecular weight approximately 19 and 31 kDa. It was concluded that the CPB gut protein patterns were changed in the effects of feeding on potato leaves treated with red kidney bean seed extract and it can be considered in the integrated pest management programs about this economical pest.
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