Geographic distribution of lactic acid bacteria in the gastrointestinal of the dwarf honey bee, Apis florea Fabricius, 1973 (Hymenoptera: Apidae)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
Probiotics are live micro-organisms that may be able to adjust microbial balance in the intestine’s host and prevent pathogen growth and colonization. Among the probiotic micro-organisms, lactic acid bacteria (LAB), which are the most common microbes hired as probiotics, are found in honey and another honey bee’s product. Probiotic bacteria boost natural microflora in host’s intestine and control pathogens’ population so that reduce risk.
The current study was carried out in 2015 and 2016 by collecting 1400 worker bees from 14 different colonies located in southern regions of Iran. The samples were put in sterile test tubes containing normal saline. The specific media were used to isolate LAB bacteria from digestive tracts of the bees. Biochemical tests and DNA extractions were done to identify the colonies. In addition, the colonies were characterized by sequencing of 16S rRNA gene using specific primers. 16S rDNA genes from 43 bacterial were sequenced. According to phylogenetic analysis, 13 different phenotypes, belonging to 6 different species including L. kunkeei, L. plantarum and L. apis, E. faecalis, E. faecium and E. hirae formed LAB flora found in gastrointestinal tract of dwarf honey bee (A. florea). Based on specific association between bacteria and A. florae, we could classify Apis populations into five groups (1: Roudan, Bandar-Abbas, Iranshahr 2. Jiroft, Kahnouj, Boushehr, Ahwaz, Dehloran 3. Gachsaran 4: Jahrom, Fassa, Behbahan and 5: Qeshm population). Furthermore, the results indicated that LABs found in digestive tracts depends on nectar and pollen found in different climates.
The current study was carried out in 2015 and 2016 by collecting 1400 worker bees from 14 different colonies located in southern regions of Iran. The samples were put in sterile test tubes containing normal saline. The specific media were used to isolate LAB bacteria from digestive tracts of the bees. Biochemical tests and DNA extractions were done to identify the colonies. In addition, the colonies were characterized by sequencing of 16S rRNA gene using specific primers. 16S rDNA genes from 43 bacterial were sequenced. According to phylogenetic analysis, 13 different phenotypes, belonging to 6 different species including L. kunkeei, L. plantarum and L. apis, E. faecalis, E. faecium and E. hirae formed LAB flora found in gastrointestinal tract of dwarf honey bee (A. florea). Based on specific association between bacteria and A. florae, we could classify Apis populations into five groups (1: Roudan, Bandar-Abbas, Iranshahr 2. Jiroft, Kahnouj, Boushehr, Ahwaz, Dehloran 3. Gachsaran 4: Jahrom, Fassa, Behbahan and 5: Qeshm population). Furthermore, the results indicated that LABs found in digestive tracts depends on nectar and pollen found in different climates.
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