Transcriptome-wide identification of Glutathione S-transferase (GST) Genes in sunn pest, Eurygaster integriceps
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
Mehdi Dastranj؛ Saeideh Asariardakani؛ Mohammad Reza ghaffari؛ Alireza Bandani؛ Ghasem Hosseini Salekdeh
چکیده
Glutathione S-transferase (GST) genes control vital traits for metabolism of the different toxins originated from plant defense system or insecticide. These detoxifying enzymes which target hydrophobic toxic compounds in phase II detoxification are widespread in both prokaryotic and eukaryotic cells. Sunn pest is the most important pest of wheat and barley in the Middle East which threat food security. Synthetic Chemical insecticide is the main approach for controlling sunn pest. Spraying more than two million hectares per season in Iran causes a great concern in public and therefore the alternative approach is Inevitable. Biotechnology based approach is a candidate for designing the highly specific bio-rational pesticide. Sequencing of sunn pest RNA provides an opportunity to identify and investigate the structure and function of the different gene family. To facilitate the identification of useful transcripts of sunn pest during feeding adult spring phase, we used an Illumina Hi-Seq 2500 protocol to sequence the whole body transcriptome. The phylogenetic tree was constructed with Neighbor Joining using the MEGA 7 and Bootstrap value based on 1,000 replications. To our knowledge, this is the first study to identify 43 GST candidate genes in sunn pest using data mining in sunn pest RNA-seq data. Then the identified candidate genes were clustered in five cytosolic GST (Delta, Theta, Zeta, Omega, and Sigma) and Microsomal GST using phylogenetic analysis. The Sigma subclasses identified as biggest subclass with 22 candidate genes, while microsomal GST identified as the smallest group with one candidate gene. The bioinformatics tools also showed that a highly conserved region with the amino acids ERVRRAHLNDLENI was present in all insect indicating its potential importance as a functional domain. Giving the role of GST in the interaction among insect, toxins, and environment, our results will provide a roadmap for future investigations on sunn pest insecticide resistance and utilize for the practical sunn pest management program.
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