16S rRNA gene based identification of gut bacteria from larvae of Ephestia kuehniella (Lepidoptera: pyralidae)

عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران

Flour moth Ephestia kuehniella Zeller is the most important pests of stored products in different countries. The pest feeding on stored products such as grains, cereals, dried fruit, almonds, hazelnuts, pistachios, etc. and the damage they do by spin a web. The use of synthetic fumigants (e.g., phosphine and methyl bromide) has been the prevailing control strategy against pest infestation in stored products. The present study describes the identification of bacteria in the gut of Ephestia kuehniella. The insect was reared under laboratory conditions on artificial diet. The gut flora of E.kuehniella was isolated on Luria Bertani Agar (LBA), followed by incubation at 28oC respectively for 48 to 72 h. The isolates were identified by using 16 SrRNA sequencing. Altogether six bacterial species of different genera were identified were Bacillus infantis, Streptomyces cacaoi, Janibacter sp., Streptomyces sp., Stenotrophomonas sp. and Acinetobacter sp., were found. The nucleotide sequences of 6 isolates were submitted to NCBI-Gen Bank and accession numbers (KX785172.1, HQ610449.1, HQ188604.1, FJ477413.1, KM576805.1 and KX066707.1) were obtained. This study provides a basis for deciding the pest control strategies.

Ephestia kuehniella؛ Endosymbiotics؛ Flour moth؛ 16S rRNA