The gut microbiota of the olive psyllids, Euphyllura straminea and Euphyllura pakistanica (Hemiptera: Aphalaridae)
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
The sap-feeding insects such as psyllids are among the most important agricultural pests. These insects harbor different groups of bacteria within their guts. The gut microbiome of insects plays an important role in their ecology and evolution, by engaging in insect fitness through participating in nutrient acquisition, immunity against natural enemies, and behavior. In the current study we investigated the gut microbiota of Euphyllura straminea and Euphyllura pakistanica (Hemiptera: Aphalaridae) which are the serious pest of olive trees in Iran by using quantitative Real-time PCR (qPCR). Different populations of E. straminea were collected from Tarom and Tehran whereas populations of E. pakistanica were collected from Shiraz. DNA was extracted from adult male and female of both species separately and subjected to the qPCR by using taxon-specific primer sets to analyze bacterial community structure at broad taxonomic levels. The selected primer sets target the major phylogenetic groups of bacteria including α-Proteobacteria (α682F and 908αR), β-Proteobacteria (Eub338F and Bet680R), γ-Proteobacteria (1080γF and γ1202R), Actinobacteria (Act920F3 and Act1200R), Firmicutes (Lgc353F and Eub518R), Bacteroidetes (798cfbF and Cfb967R) and Universal bacterial (906F and 1062R). Our results indicated that bacterial taxa within the phylum Proteobacteria were dominant in the all populations of E. straminea and E. pakistanica. γ-Proteobacteria were the most abundant bacteria in the psyllids followed by α-Proteobacteria, β-Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, respectively. Also, the results showed that female psyllids harbored greater populations of the gut bacteria compared with the males. Interestingly, the bacterial community structure was similar in the populations of both species. These results showed that the bacterial community is important in biology of these insects so that different geographical populations of these insects harbor similar bacterial communities. Our study provides fundamental information for future investigation on these economically-important insect pests and psyllid-symbiont interactions.
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