Evaluation Larvicidal Activity of Coconut Oil against Anopheles stephensi the Main Vector of Malaria in Iran
عنوان دوره: دومین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
Introduction: Continuous uses of chemical larvicides cause resistance in many species around the world and also environmental pollution. Herbal larvicides are suitable alternatives for this purpose.
METHODS: Different concentrations of Coconut Oil were prepared (i.e. 6.25 – 300 ppm) by diluting with ethanol. The larvicidal activity of Coconut Oil was tested against 4th instars larvae of laboratory-reared Anopheles stephensi (obtained from department of medical entomology, Tehran University of Medical sciences), based on WHO guideline. Tests were repeated 12 times in 3 different replicates in recommended condition, including 25-28°C and 12h L: 12h D photoperiod.
RESULTS: The bioassay of oil were performed, LC (lethal concentration) at 50 & 90% with values of 56.67 and 107.072 were calculated using probit analysis. No mortality in controls was observed (with adding ethanol).
CONCLUSION: The use of botanical larvicide may help in reducing the environmental side effects of synthetic insecticides. And also reduce the occurrence of resistance.
METHODS: Different concentrations of Coconut Oil were prepared (i.e. 6.25 – 300 ppm) by diluting with ethanol. The larvicidal activity of Coconut Oil was tested against 4th instars larvae of laboratory-reared Anopheles stephensi (obtained from department of medical entomology, Tehran University of Medical sciences), based on WHO guideline. Tests were repeated 12 times in 3 different replicates in recommended condition, including 25-28°C and 12h L: 12h D photoperiod.
RESULTS: The bioassay of oil were performed, LC (lethal concentration) at 50 & 90% with values of 56.67 and 107.072 were calculated using probit analysis. No mortality in controls was observed (with adding ethanol).
CONCLUSION: The use of botanical larvicide may help in reducing the environmental side effects of synthetic insecticides. And also reduce the occurrence of resistance.
کلیدواژه ها