Effect of Cydia pomonella granulovirus (CpGV) on some biochemical parameters of codling moth, Cydia pomonella(L.)
عنوان دوره: اولین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
The CpGV is one of the mortality factors that can be reduced population of Cydia pomonella (L.) throughtout the world. Recently, some informal reports refer the reduction of acute effects of CpGV. So, in this research aimed the evaluation of the chronic effects of CpGV on some bioenergetic resources, as the key factors in overwintering of C. pomonella. This virus can infect the larvae immediately before entering into the fruit. So, the larvae may be weakened and the fat bodies may be destroyed by a virus. In this research were examined the side effects of concentration levels 1,2and 4OB/µl of CpGV after 7th day on the mount of protein and glycogen on 5th instar C. pomonella according to Yee & Chapman and Van Handel method respectively. Larvae been reared on artificial diet and in incubated with light(16:8) (D:L), relative humidity of 10±60 percent and temperature conditions2±24 c˚ . Larvae were infected via impregnated artificial food with specified concentrations virus. Results showed that CpGV decreased total protein and glycogen (p<0.001), significantly. The average of the amount of protein in concentrations 1,2 and 4 OB/µl and control was 9.74±0.74, 8.57±0.88, 0.81±0.008 and 11.80±1.16 mg/g fresh weight, respectively. Also, the amount of glycogen was 7.5±0.48, 0.67±0.11, 2.87±0.95 and 3.28±1 mg/g fresh weight, respectively. The results indicated that CpGV can decrease some of bioenergetic resources of the larvae and they may be weakened. According to the influence of energy resources on the survival rate of overwintering insect, this virus may be disrupted overwintering of colding moth.
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