Identification and isolation of the acidophilus bacterium of genus Acetobacter in migut of Culex mosquitoes in Iran as a paratransgenic tool

عنوان دوره: اولین کنگره بین المللی حشره شناسی ایران

Mosquitoes of the Culex pipiens are participated in the transmission of several human pathogens, such as West Nile virus, Saint Louis encephalitis virus and some filarial worms, as well as wildlife pathogens such as the avian malaria parasite. Investigations of mosquito’s microbiota continue to broaden the span of possible Normal flora-based applications against vector-borne diseases. In this study, genus Acetobacter as a α-proteobacteria is emerging as possible endoflora candidates, Especially in Paratransgenic approaches purposed at blocking pathogen transmission. Here, we sum up an introductory review of laboratory-reared Cx. pipiens for the presence of Acetobacter spp., and provide the first isolation and identification of Acetobacter in some of the members of the Cx. pipiens in Iran. Mosquitoes were obtained from colonies of larvae, pupae and adult Culex. The enrichment culture advance CaCO3 broth was engaged for the isolation of acetic acid bacteria. Acetobacter was successfully isolated and identified from Culex. Acetobacter spp. that was isolated from mosquitoes of all the strains analyses on selected medium CaCO3 agar; their identity was confirmed by PCR of genomic DNA extracted from single colonies using Acetobacter-specific primers. Nucleotide sequences were aligned with available sequences for representative of Acetobacter Aceti, Acetobacter cerevisiae, Acetobacter cibinongensis, Acetobacter diazotrophicus, Acetobacter estunensis, Acetobacter europaeus and Acetobacter fabarum. Phylogeny analysis revealed position of the isolated Acetobacter among other species. The successful isolation of Acetobacter in this study, promise as useful candidate for vector control with Paratransgenesis tool.

Acetobacter؛ Culex؛ Paratransgenesis؛ microbiota