Virulence of Metarhizium anisopliae on Callosobruchus maculatus (F.) (Col.: Bruchidae) larvae in stored cowpea
عنوان دوره: اولین کنگره بین المللی حشره شناسی ایران
نویسندگان
چکیده
Virulence of the entomopathogenic fungus; Metarhizium anisopliae (isolates: M14 and DEMI001) was studied against third instar larvae of Callosobruchus maculatus (F.) (Col.: Bruchidae) by immersion method. For each isolates, 5 aqueous suspensions (104-108 conidia/ml) were prepared using Tween 80 (0.02% v/v). Results revealed that third instar larvae of this bruchid pest were susceptible to both isolates of M. anisopliae. The mortality percents of larvae increased with increasing conidial concentration. The cumulative mortality percent of C. maculatus larvae ranged from 6.67-96.67% and 3.33-93.33% for M14 and DEMI001 isolates at different conidial concentration, respectively, 10 days after inoculation. Probit analysis demonstrated overlaping of 95% confidence limits of LC50 and LC95 and significant differences weren’t observed among two isolates. The corresponding LC50 values were 1.15×106 and 1.63×106 (conidia/ml) for M14 and DEMI001 isolates, respectively, while the LT50 values were 5.45 and 6.68 days. The mean comparisions for cumulative mortality percent at different conidial concentrations indicated that the mortality rates were not affected significantly by different fungus isolate at any conidial concentration. The results of this study highlighted the importance of these M14 and DEMI001 isolates for the biological control of C. maculatus.
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